Preparation of recombinant firefly luciferase by a simple and rapid expression and purification method and its application in bacterial detection

A simple and rapid expression and purification method of recombinant firefly luciferase was developed for bacteria detection. A modified luciferase gene from North American firefly Photinus pyralis was cloned into pET28a expression vector and the recombinant protein was produced in Escherichia coli BL21. The recombinant luciferase, equipped with a polyhistidine affinity tag, was purified by immobilized metal ion affinity chromatography (IMAC). The approach generated an abundant expression and an efficient purification of a recombinant luciferase with final yield 1.995mg/L of cell culture. Experiments on the recombinant luciferase also showed that the relative light units (RUL) of the enzyme were 5.8×108, and the specific activity was 2.9×1010 RLU/mg. By applying adenosine triphosphate (ATP) bioluminescence to detection of the coin bacteria using the recombinant protein, the ATP content of bacteria was 9.48×10-16mol/mL, and was identical to the bacteria counts (4500CFU/mL) in order of magnitude. Taken together, our results provided a simple and efficacious method of the preparation of recombinant luciferase, which could be applied in the determination of bacteria via ATP bioluminescence.     

图像边界检测区域对比度模糊增强算法在轮廓提取中的运用

分析了图像边界检测的区域对比度模糊增强算法的特点，通过实验验证了该算法的正确性和可行性，并指出了算法的一些不足之处和待改进的地方，提出了一种适用轮廓提取的新广义算子。     

高桩码头结构承载力检测与评估方法

既有码头结构在受到不同情况的损伤或者需要升级改造的情况下,需要对其结构承载力进行检测评估,现有研究主要针对旧码头材料耐久性进行研究,很少涉及结构整体承载力研究。通过对大量旧码头结构的调查研究,分析了高桩码头结构常见的损坏原因,总结了高桩码头结构承载检测评估的特点,对已建高桩码头结构承载力检测与评估的前提条件、基本程序以及检测与评估的内容等做了详细的技术说明,为正确进行高桩码头检测评估提供了一套可行的分析方法。     

汽车维修中检测诊断技术的应用探讨

现如今汽车已经成为人们必不可少的代步工具,汽车的数量随之也越来越多,在汽车的行驶过程中,难免会出现故障之类的问题,因此汽车的维修工作也变的尤为重要,其维修的师傅必须具备丰富的工作经验以及理论知识,然后借助检测诊断技术来进行汽车的事故和问题故障的检测排查工作。本文围绕汽车的检测诊断技术以及该技术在汽车维修中的应用展开分析。     

基于STM32的蓄电池内阻测量系统设计

文章设计了一款以STM32微处理器为核心的蓄电池内阻测量系统,采用交流阻抗法对电池内阻进行测量。针对测量信号微弱,易淹没于噪声的特点,应用相关检测技术以检出有用信息。调试和对比实验数据表明,该系统可实现对蓄电池内阻的在线、快速、精准测量。     

LPG船舷侧结构的碰撞性能研究

研究了LPG船舷侧结构碰撞损伤过程和多种构件的抗撞作用，通过分析发现，LPG船的舷侧耐撞力远远低于同吨位的常规单壳船。LPG船的强肋骨在抵抗碰撞中起主要作用，由此提出提高常规LPG船舷侧结构提高耐撞力的最佳途径。     

Efficient multiple model particle filtering for joint traffic state estimation and incident detection

This article proposes an efficient multiple model particle filter (EMMPF) to solve the problems of traffic state estimation and incident detection, which requires significantly less computation time compared to existing multiple model nonlinear filters. To incorporate the on ramps and off ramps on the highway, junction solvers for a traffic flow model with incident dynamics are developed. The effectiveness of the proposed EMMPF is assessed using a benchmark hybrid state estimation problem, and using synthetic traffic data generated by a micro-simulation software. Then, the traffic estimation framework is implemented using field data collected on Interstate 880 in California. The results show the EMMPF is capable of estimating the traffic state and detecting incidents and requires an order of magnitude less computation time compared to existing algorithms, especially when the hybrid system has a large number of rare models.     

斗轮式取料机行走位置精确定位

斗轮式取料机行走位置校正装置在定位中起到很重要的作用.分析了原有行走机构定位原理与状况.介绍了新定位装置的设计思路和实施方案,改革后提高了全场设备相互协同的效率和相互保护的可靠性.     

主动声纳的低截获性数字仿真研究

在现代海战中,舰艇面临着越来越严峻的敌方侦察声纳的威胁.低截获是一项非常诱人的技术.低截获声纳通过选择合适的发射信号,在保持探测能力的同时,降低被敌方侦察声纳截获的概率.采用数字仿真的形式,分析浅海侦察声纳信号能量的变化,研究主动声纳的低截获性.仿真结果表明在高斯噪声背景下,主动声纳实现低截获性相当困难.     

交通运输超限治理对策研究

由于交通运输量的不断增大以及运输行业的恶性竞争等原因，导致货车超载现象越来越普遍，并已成为急需解决的问题。探讨交通运输超限治理的对策，对于完善科学而系统的超限管理体系具有一定的借鉴意义。